Human and Translational Immunology Lab
In our working group we investigate the immune response generated in different physiological and pathological situations in humans.
To this end, we use several experimental strategies:
- a) phenotypic characterisation of a single immune cell, either circulating in blood or tissue, by multiparametric flow cytometry and subsequent analysis of the data by unsupervised computational methods;
- b) animal models of human pathologies to investigate immunological mechanisms or test hypotheses;
- c) spatial relationships between immune cells through digital multiplex immunohistochemistry and
- d) computational analysis of "omics" data already published and residing in public banks.
Our projects focus on: -the study of the immune response generated in children with Down's syndrome, analysing T cells, B cells and dendritic cells in tonsils removed for obstructive sleep apnoea. the immunological characteristics of the tumour microenvironment in murine models of cancer treated with different TLR ligands - the computational analysis of immunological data from cancer patients treated with responder and non-responder immunocheckpoints - the computational analysis of immunological data from triple-negative breast cancer patients treated with anti-GGEF and anti PDL-1 or anti-GGEF alone.
In 1997 she carried out a research stay at the Necker-Enfants Malades Hospital through the INSERM-CONICET agreement. Subsequently, between 1998 and 2000, she did her postdoctoral work at the Institut de Genetique et Biologie Moleculaire et Celullaire (Strasbourg, France) under the direction of Dr. Diane Mathis and Dr. Christoph Benoist.
Since 2001 she has been a member of the CONICET Research Career, where she is currently a Principal Researcher at CIBICI.
In addition, she teaches at the Department of Clinical Biochemistry FCQ-UNC, holding the position of Associate Professor.
In 2013-2017 he did his first postdoctoral work at the Institut Curie, Laboratoire de Transfert INSERM U932 – Paris, France, under the direction of Dr. Eliane Piaggio. Her research covered Immunology and cancer immunotherapy Human breast cancer – T, myeloid, B, DC.
In 2016 she participated in an Exchange Programme at Novartis Biologics Center (Basel, Switzerland), under the direction of Dr. E. Traggiai. Her work there was focused on the purification and cloning of B cell subsets. Subsequently, she did a second postdoctoral work between 2017 and 2022, at the Institute for Experimental Immunology. Universität Zürich (UZH) (Zurich, Switzerland), under the direction of Dr. Burkhard Becher. There, he worked in depth on Cancer Immunology and Immunotherapy: Human Tumors – high dimensional cytometry and bioinformatics.
Since 2021 he has been a member of the CONICET Research Career, where he is currently an Assistant Researcher at CIBICI.
In addition, he teaches at the Department of Clinical Biochemistry FCQ-UNC, holding the position of Associate Professor.
She is a CONICET Doctoral Fellow, under the direction of Dr. Mariana Maccioni. She is doing her PhD in Chemical Sciences at the FCQ-UNC, and her thesis deals with the study «Study of prognostic and predictive markers in cancers».
She is a CONICET Doctoral Fellow, under the direction of Dr. Mariana Maccioni. She is doing her PhD in Chemistry at the FCQ-UNC, and her thesis is on the topic: «Search for predictive biomarkers of response to Immune Checkpoint Inhibitors (ICIs) in peripheral blood samples».
She is also a lecturer in the Department of Clinical Biochemistry FCQ-UNC, holding the position of Assistant Professor.
He is a CONICET Doctoral Fellow, under the direction of Dr. Mariana Maccioni. He is doing his PhD in Chemical Sciences at the Faculty of Chemical Sciences (FCQ-UNC), and his thesis deals with the topic: «Analysis of the immune compartment in individuals with trisomy 21: functional implications».
He also teaches in the Department of Clinical Biochemistry FCQ-UNC, occupying the position of Assistant Professor.
Ana Paula Flores Guirado
Student
Student
Collaborators and Partners
Collaborators and Partners
Joaquín Espinosa is Executive Director of the Linda Crnic Down Syndrome Institute. Department of Pharmacology. University of Colorado Denver.
Eliane Piaggio is Director of the Translational Immunotherapy Unit of the Immunity and Cancer Unit at INSERM, Institut Curie.
Silvia Lopez De Blanc is with the Department of Oral Pathology, Chair of Stomatology, Faculty of Dentistry, National University of Cordoba, Cordoba, Argentina.
Rosana Morelatto is with the Department of Oral Pathology, Chair of Stomatology, Faculty of Dentistry, National University of Córdoba, Córdoba, Argentina.
Ana María Ferreira Vázquez is Associate Professor of Immunology at the University of the Republic of Uruguay.
Selected Publications
Distinct immune signatures discriminate SARS-CoV-2 vaccine combinations. Nicolás Gonzalo Núñez*&, Jonas Schmid*, Laura Power*, Chiara Alberti, Sinduya Krishnarajah+, Stefanie Kreutmair+, Susanne Unger+, Sebastián Blanco, Brenda Konigheim, Constanza Marín, Luisina Onofrio, Jenny Christine Kienzler, Sara da Costa Pereira, Florian Ingelfinger, InmunoCovidCba, InViV working group , Marina E. Pasinovich, Juan M Castelli, Carla Vizzotti, Maximilian Schaefer, Juan Villar-Vesga, Carla Helena Merten, Aakriti Sethi, Tobias Wertheimer, Mirjam Lutz, Danusia Vanoaica, Claudia Sotomayor, Adriana Gruppi, Christian Münz, Diego Cardozo, Gabriela Barbás, Laura Lopez, Paula Carreño, Gonzalo Castro, Elias Raboy, Sandra Gallego#, Gabriel Morón#, Laura Cervi#, Eva V Acosta Rodriguez#, Belkys A Maletto#, Mariana Maccioni#&, Burkhard Becher*#. & Corresponding authors. Nature Immunology. 2023 Feb. In press
Trisomy 21 dysregulates T cell lineages toward an autoimmunity-prone state associated with interferon hyperactivity. Paula Araya, Katherine A Waugh, Kelly D Sullivan, Nicolás G Núñez, Emiliano Roselli, Keith P Smith, Ross E Granrath, Angela L Rachubinski, Belinda Enriquez Estrada, Eric T Butcher, Ross Minter, Kathryn D Tuttle, Tullia C Bruno, Mariana Maccioni, Joaquín M Espinosa. Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24231-24241. doi: 10.1073/pnas.1908129116.
TLR3 Activation of Intratumoral CD103 + Dendritic Cells Modifies the Tumor Infiltrate Conferring Anti-tumor Immunity. Emiliano Roselli, Paula Araya, Nicolás Núñez, Gerardo Gatti, Francesca Graziano, Christine Sedlik, Philippe Benaroch, Eliane Piaggio, Mariana Maccioni. Front Immunol. 2019 Mar 20;10:503. doi: 10.3389/fimmu.2019.0050
Oral squamous cell carcinoma (OSCC) tumors from heavy alcohol consumers are associated with higher levels of TLR9 and a particular immunophenotype: Impact on patient survival. Nicolás Bolesina, Gerardo Gatti, Silvia López de Blanc, Sabrina Dhooge, Darío Rocha, Elmer Fernandez, Ruth Ferreyra, Vanesa Palla, Verónica Grupe , Rosana Morelatto, Mariana Maccioni. Front Immunol. 2022 Aug 5;13:941667. doi: 10.3389/fimmu.2022.941667. eCollection 2022
High IRF8 expression correlates with CD8 T cell infiltration and is a predictive biomarker of therapy response in ER negative breast cancer. Gerardo Gatti, Courtney Betts, Darío Rocha, Maribel Nicola, Verónica Grupe, Cecilia Ditada; Nicolas G. Nuñez; Emiliano Roselli; Paula Araya, Jeremías Dutto, Lucia Boffelli, Elmer Fernández, Lisa M. Coussens, MARIANA MACCIONI
Immune signatures predict development of autoimmune toxicity in immune checkpoint inhibitor-treated patients with cancer. Nicolas Gonzalo Nuñez*, Fiamma Berner*, Ekaterina Friebel*, Susanne Unger, Nina Wyss,, Julia Martinez Gomez, Mette-Triin Purde, Rebekka Niederer,, Maximilian Porsch, Christa Lichtensteiger, Rafaela Kramer, Michael Erdmann, Christina Schmitt, Lucie Heinzerling, Marie-Therese Abdou, Julia Karbach, Dirk Schadendorf, Lisa Zimmer, Selma Ugurel, Niklas Klümper, Michael Hölzel,, Laura Power, Stefanie Kreutmair, Mariaelena Capone, Gabriele Madonna, Lacin Cevhertas, Anja Heider, Teresa Amaral, Omar Hasan Ali, David Bomze, Florentia Dimitriou, Stefan Diem, Paolo Antonio Ascierto, Reinhard Dummer, Elke Jäger, Christoph Driessen, Mitchell Paul Levesque, Willem van de Veen, Markus Joerger, Martin Früh, Burkhard Becher**, Lukas Flatz **. Med (N Y). 2023 Jan 13;S2666-6340(22)00523-2. doi: 10.1016/j.medj.2022.12.007.
NASH limits anti-tumor surveillance in immunotherapy-treated HCC. Dominik Pfister, Nicolás Gonzalo Núñez, Roser Pinyol, Olivier Govaere, Matthias Pinter, Marta Szydlowska, Revant Gupta, Mengjie Qiu, Aleksandra Deczkowska, Assaf Weiner, Florian Müller, Ankit Sinha, Ekaterina Friebel, Thomas Engleitner, Daniela Lenggenhager, Anja Moncsek, …….. Mathias Heikenwalder. Nature. 2021 Mar 24. doi: 10.1038/s41586-021-03362-0.
Unique immunological signatures distinguish severe COVID-19 from non-SARS-CoV-2-driven critical pneumonia. Stefanie Kreutmair; Susanne Unger; Nicolás Gonzalo Núñez; Florian Ingelfinger; Chiara Alberti; Donatella De Feo; Sinduya Krishnarajah; Kauffmann Manuel; Ekaterina Friebel; Benjamin Gaborit; Sepideh Babaei; Mirjam Lutz; Nicole Puertas Jurado; Nisar P. Malek; Siri Goepel; Peter Rosenberger; Helene A. Häberle; Ikram Ayoub; Sally Al-Hajj; Manfred Classen; Roland Liblau; Guillaume Martin-Blondel; Michael Bitzer; Antoine Roquilly; Burkhard Becher. Immunity. 2021 doi: https:// doi.org/10.1016/j.immuni.2021.05.002.
Tumor invasion in draining lymph nodes is associated with Treg accumulation in breast cancer patients. Nicolas Gonzalo Núñez, Jimena Tosello Boari, Rodrigo Nalio Ramos, Wilfrid Richer, Nicolas Cagnard, Cyrill Dimitri Anderfuhren, Leticia Laura Niborski, Jeremy Bigot, Didier Meseure, Philippe De La Rochere, Maud Milder, Sophie Viel, Delphine Loirat, Louis Pérol, Anne Vincent-Salomon, Xavier Sastre-Garau, Becher Burkhard, Christine Sedlik, Olivier Lantz, Sebastian Amigorena, Eliane Piaggio. Nat Commun. 2020 Jun 29;11(1):3272. doi: 10.1038/s41467-020-17046-2. *These authors contributed equally.